PCR-based markers for genetic mapping in Neurospora crassa.

Rapid genetic mapping in Neurospora crassa.

Forward genetic analysis is the most broadly applicable approach to discern gene functions. However, for some organisms like the filamentous ascomycete Neurospora crassa, genetic mapping frequently represents a limiting step in forward genetic approaches. We describe an efficient method for genetic mapping in N. crassa that makes use of a modified bulked segregant analysis and PCR-based molecul...

Fixed genetic instability in Neurospora crassa.

NE of the most convincingly documented genetic phenomena is the great Ovariabi l i t y in spontaneous mutation frequencies at different genetic sites. This variability is seen most readily in BENZER’S topographic maps of the rII region of phage T4 in which the linear distributions of mutants of different origins are compared (BENZER 1961). Restricting our attention to spontaneously arising muta...

Genetic and Non-Genetic Factors in Pigmentation of Neurospora Crassa.

HE biochemical genetics of carotenoid pigments has been studied in the T tomato ( LEROSEN et al. 1941 ; MACKINNEY and JENKINS 1949 ; PORTER and LINCOLN 1950), corn (MANGELSDORF and FRAPS 1931) and red yeast (BONNER et al. 1946). HAXO (1949) worked out the carotenoid components of the wild type Neurospora. The present paper deals with genetic and biochemical studies of a modifier system for pigm...

Quelling in Neurospora crassa.

Heterocaryosis in Neurospora Crassa.

T IS possible by means of X-ray or ultraviolet treatment to produce mutant I strains of Neurospora crassa that are deficient in their abilities to synthesize specific vitamins, amino acids, or other essential substances (BEADLE and TATUM 1941; TATUM and BEADLE 1g4zb). It occurred to us that it might be possible to determine whether the mutant genes concerned in such haploid strains are recessiv...